Induction of reactive oxygen species-stimulated distinctive autophagy by chelerythrine in non-small cell lung cancer cells.
Identifieur interne : 000D12 ( Main/Exploration ); précédent : 000D11; suivant : 000D13Induction of reactive oxygen species-stimulated distinctive autophagy by chelerythrine in non-small cell lung cancer cells.
Auteurs : Zheng-Hai Tang [République populaire de Chine] ; Wen-Xiang Cao [République populaire de Chine] ; Zhao-Yu Wang [République populaire de Chine] ; Jia-Hong Lu [République populaire de Chine] ; Bo Liu [République populaire de Chine] ; Xiuping Chen [République populaire de Chine] ; Jin-Jian Lu [République populaire de Chine]Source :
- Redox biology [ 2213-2317 ] ; 2017.
Descripteurs français
- KwdFr :
- Antinéoplasiques (pharmacologie), Autophagie, Benzophénanthridines (pharmacologie), Carcinome pulmonaire non à petites cellules (métabolisme), Cellules A549, Espèces réactives de l'oxygène (métabolisme), Humains, Lignée cellulaire tumorale, Prolifération cellulaire (), Protéines associées aux microtubules (métabolisme), Relation dose-effet des médicaments, Régulation de l'expression des gènes tumoraux (), Survie cellulaire (), Tumeurs du poumon (métabolisme).
- MESH :
- métabolisme : Carcinome pulmonaire non à petites cellules, Espèces réactives de l'oxygène, Protéines associées aux microtubules, Tumeurs du poumon.
- pharmacologie : Antinéoplasiques, Benzophénanthridines.
- Autophagie, Cellules A549, Humains, Lignée cellulaire tumorale, Prolifération cellulaire, Relation dose-effet des médicaments, Régulation de l'expression des gènes tumoraux, Survie cellulaire.
English descriptors
- KwdEn :
- A549 Cells, Antineoplastic Agents (pharmacology), Autophagy, Benzophenanthridines (pharmacology), Carcinoma, Non-Small-Cell Lung (metabolism), Cell Line, Tumor, Cell Proliferation (drug effects), Cell Survival (drug effects), Dose-Response Relationship, Drug, Gene Expression Regulation, Neoplastic (drug effects), Humans, Lung Neoplasms (metabolism), Microtubule-Associated Proteins (metabolism), Reactive Oxygen Species (metabolism).
- MESH :
- chemical , metabolism : Microtubule-Associated Proteins, Reactive Oxygen Species.
- chemical , pharmacology : Antineoplastic Agents, Benzophenanthridines.
- drug effects : Cell Proliferation, Cell Survival, Gene Expression Regulation, Neoplastic.
- metabolism : Carcinoma, Non-Small-Cell Lung, Lung Neoplasms.
- A549 Cells, Autophagy, Cell Line, Tumor, Dose-Response Relationship, Drug, Humans.
Abstract
Chelerythrine (CHE), a natural benzo[c]phenanthridine alkaloid, shows anti-cancer effect through a number of mechanisms. Herein, the effect and mechanism of the CHE-induced autophagy, a type II programmed cell death, in non-small cell lung cancer (NSCLC) cells were studied for the first time. CHE induced cell viability decrease, colony formation inhibition, and apoptosis in a concentration-dependent manner in NSCLC A549 and NCI-H1299 cells. In addition, CHE triggered the expression of phosphatidylethanolamine-modified microtubule-associated protein light-chain 3 (LC3-II). The CHE-induced expression of LC3-II was further increased in the combination treatment with chloroquine (CQ), an autophagy inhibitor, and large amounts of red-puncta were observed in the CHE-treated A549 cells with stable expression of mRFP-EGFP-LC3, indicating that CHE induces autophagy flux. Silence of beclin 1 reversed the CHE-induced expression of LC3-II. Inhibition of autophagy remarkably reversed the CHE-induced cell viability decrease and apoptosis in NCI-H1299 cells but not in A549 cells. Furthermore, CHE triggered reactive oxygen species (ROS) generation in both cell lines. A decreased level of ROS through pretreatment with N-acetyl-L-cysteine reversed the CHE-induced cell viability decrease, apoptosis, and autophagy. Taken together, CHE induced distinctive autophagy in A549 (accompanied autophagy) and NCI-H1299 (pro-death autophagy) cells and a decreased level of ROS reversed the effect of CHE in NSCLC cells in terms of cell viability, apoptosis, and autophagy.
DOI: 10.1016/j.redox.2017.03.009
PubMed: 28288416
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">Chelerythrine (CHE), a natural benzo[c]phenanthridine alkaloid, shows anti-cancer effect through a number of mechanisms. Herein, the effect and mechanism of the CHE-induced autophagy, a type II programmed cell death, in non-small cell lung cancer (NSCLC) cells were studied for the first time. CHE induced cell viability decrease, colony formation inhibition, and apoptosis in a concentration-dependent manner in NSCLC A549 and NCI-H1299 cells. In addition, CHE triggered the expression of phosphatidylethanolamine-modified microtubule-associated protein light-chain 3 (LC3-II). The CHE-induced expression of LC3-II was further increased in the combination treatment with chloroquine (CQ), an autophagy inhibitor, and large amounts of red-puncta were observed in the CHE-treated A549 cells with stable expression of mRFP-EGFP-LC3, indicating that CHE induces autophagy flux. Silence of beclin 1 reversed the CHE-induced expression of LC3-II. Inhibition of autophagy remarkably reversed the CHE-induced cell viability decrease and apoptosis in NCI-H1299 cells but not in A549 cells. Furthermore, CHE triggered reactive oxygen species (ROS) generation in both cell lines. A decreased level of ROS through pretreatment with N-acetyl-L-cysteine reversed the CHE-induced cell viability decrease, apoptosis, and autophagy. Taken together, CHE induced distinctive autophagy in A549 (accompanied autophagy) and NCI-H1299 (pro-death autophagy) cells and a decreased level of ROS reversed the effect of CHE in NSCLC cells in terms of cell viability, apoptosis, and autophagy.</div>
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